Isolation of Natural Inhibitors of Papain Obtained from Carica papaya Latex
نویسندگان
چکیده
Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kine tics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P -III).
منابع مشابه
استخراج، شناسایی و بررسی فعالیت آنزیم پاپائین میوه Carica papaya از ایران
Background and purpose: Papain, a proteolytic enzyme, is found in the dried and purified latex from the fruits of Carica papaya L. (Caricaceae). Papain is used in medicine as a digestive and in the debridement of necrotic tissues. It is used in pharmaceutics as a spreading agent for drugs, supplied as an ointment (10%) with urea which denatures nonviable protein matter present in lesions. ...
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